Construction of a Vector for the Surface Display of Heterologous Proteins in Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Construction of a sorbitol-based vector for expression of heterologous proteins in Saccharomyces cerevisiae.
A new inducible yeast expression vector, pXS7, was constructed by using the promoter and terminator sequences from the Saccharomyces cerevisiae SOR1 gene, which codes for the sorbitol dehydrogenase protein. We cloned the coding sequence of the Saccharomyces YEF3 gene in this vector and demonstrated an increase in YEF3 protein levels when cells were grown in the presence of the sugar sorbitol.
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ژورنال
عنوان ژورنال: Research Journal of Microbiology
سال: 2012
ISSN: 1816-4935
DOI: 10.3923/jm.2012.41.49